The man allegedly ran over two Israeli forces on Route 60 near the illegal settlement of Shilo, with Israeli forces shooting the suspect at the scene.
Israeli media reported that two Israelis were seriously wounded and another lightly injured in the attack, without specifying whether they were civilians or military personnel.
Israeli soldiers opened fire at the car, with the suspect said to be seriously injured after the vehicle overturned.
The unidentified suspect was reportedly treated at the scene, although his condition is unknown.