The protesters were marching Wednesday to denounce the police shooting of African American Keith Lamont Scott, killed in the city late on Tuesday.
The 43-year-old’s death outraged Black Lives Matters activists, leading to a violent protest there.
"No Justice, No Peace," shouted the demonstrators as they clashed with heavily-armed police officers in riot gear.
There were conflicting reports about how Scott was killed with police alleging that he was armed, ignored police order, and hurt 16 officers.
Charlotte Police Chief Kerr Putney said a protester reported earlier as heavily injured and transferred to hospital has died.
Police later claimed that the protester was not shot by police at all, the Associate Press reported.
Police was using rubber bullets and tear gas to put an end to the second night of the protests in the eastern state.
The Charlotte Metropolitan Police Department declared the protests an "unlawful assembly" and announced their willingness to use "non-lethal instruments" to control the crowd, estimated to be in the hundreds, RT reported.
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