Moner Mohammad Abu Salha, alias Abu Hurayra al-Amriki, was believed to be the first American national to carry out such an attack in Syria's more than three years of war.
"I want to rest in the afterlife, not in this world... My heart is not at peace here. Hopefully it will be in heaven," Abu Hurayra claims in broken Arabic in the 17-minute video posted on YouTube on Friday by Al-Nusra Front.
The footage, released via Al-Nusra's official channel Al-Manara Al-Baydaa, also shows Abu Hurayra saying: "I came to Syria without money to buy a rifle or a pouch.”
The American extremist carried out a May 25 truck bomb attack on an army base in Jabal al-Arbaeen area of northwest Syria's Idlib province.
Six days later, the State Department confirmed that the US citizen, who travelled to Syria in 2013, had carried out a suicide attack.
The parents of "the Florida boy" own a grocery store, with his father from Jordan and mother a convert to Islam, US newspapers reported.
Estimates of the number of foreign fighters who have flooded into Syria in the past three years range from between 9,000 to 11,000.
The video gives few details about Abu Hurayra's precise background, but shows him alongside three other suicide bombers, seated near an Al-Nusra black flag, each speaking of their mission.
At the time, the opposition-linked Syrian Observatory for Human Rights said four suicide attacks on army positions in Idlib on May 25 left dozens of casualties.
The video says Abu Hurayra attacked an army command building, while an extrimist from the Maldives was also among the four bombers.
NTJ/MB